mouse antibody mab1696 Search Results


90
R&D Systems mab1696
CD93 expression and absolute numbers on the various B-cell developmental subsets in BM and peripheral lymphoid organs. a The first column divides lymphoid-gated B cells in the bone marrow into mature recirculating (B220 hi /IgM+), immature (B220 lo /IgM+), and pro-/pre-B cells (B220 lo /IgM−). The histograms resolve CD93 expression by AA4.1 antibody on pro-/pre- and immature B cells ( black line ) in contrast with mature recirculating B cells ( dashed line ). The ratio of the mean fluorescence indices (MFIs) of AA4.1 expression by pro-/pre- and immature B cells to the AA4.1 expression by mature recirculating B cells is shown as MFI fold Δ on the histograms. Splenic B-cell developmental subsets are divided into the marginal zone (MZ) pool (B220+ gated, IgM hi /CD21/35 hi ), FO pool (B220+ gated, IgM int /CD21/35 int ), and TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the FO pool ( dashed line ). The ratio of MFIs of AA4.1 expression by TR B cells and FO B cells is shown as MFI fold Δ . B cells in the peripheral blood are divided into the mature recirculating (B220+ gated, IgM+/CD21/35 int ) and the TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the mature recirculating pool ( dashed line ). The MFI fold Δ is an average of at least n = 5 mice per strain shown, and * p < 0.05 compared with B6 mice. b The left panel shows the total numbers of AA4.1+ pro-/pre- and immature B cells in the BM of B6 ( n = 12; black bar ) and NOD ( n = 10; white bar ) and NZB/W F1 ( n = 5, gray bar ) mice. The middle panel shows the total number AA4.1+ TR B cells in the spleen of B6 ( n = 9; black bar ), NOD ( n = 9; white bar ), and NZB/W F1 ( n = 5; gray bar ) mice. The right panel shows the percent AA4.1+ cells in the pro-/pre- and immature B cells in the BM and the TR B-cell compartments in the PBL and spleen of B6 ( n = 12 for BM compartments, n = 9 for spleen, n = 5 for PBL), NOD ( n = 10 for BM compartments, n = 9 for spleen, n = 5 for PBL), and NZB/W F1 ( n = 5 for all compartments) mice. Error bars indicate ± SD, ** p < 0.01 compared with B6 mice. c The column on the left shows characteristic AA4.1 expression of B220 cells in the BM of a B6, NOD, and NZB/W F1 mouse. On the right , AA4.1 and 493 Abs are used in conjunction on B220+ gated lymphocytes in the BM. The histograms show expression of <t>mAb1696</t> by pro-/pre- and immature ( black line ) in contrast with mature recirculating ( dashed line ) B cells in the BM of B6, NOD, and NZB/W F1 mice. Staining is representative of at least n = 5 per mouse strain
Mab1696, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
GenScript corporation mouse antibody mab1696
CD93 expression and absolute numbers on the various B-cell developmental subsets in BM and peripheral lymphoid organs. a The first column divides lymphoid-gated B cells in the bone marrow into mature recirculating (B220 hi /IgM+), immature (B220 lo /IgM+), and pro-/pre-B cells (B220 lo /IgM−). The histograms resolve CD93 expression by AA4.1 antibody on pro-/pre- and immature B cells ( black line ) in contrast with mature recirculating B cells ( dashed line ). The ratio of the mean fluorescence indices (MFIs) of AA4.1 expression by pro-/pre- and immature B cells to the AA4.1 expression by mature recirculating B cells is shown as MFI fold Δ on the histograms. Splenic B-cell developmental subsets are divided into the marginal zone (MZ) pool (B220+ gated, IgM hi /CD21/35 hi ), FO pool (B220+ gated, IgM int /CD21/35 int ), and TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the FO pool ( dashed line ). The ratio of MFIs of AA4.1 expression by TR B cells and FO B cells is shown as MFI fold Δ . B cells in the peripheral blood are divided into the mature recirculating (B220+ gated, IgM+/CD21/35 int ) and the TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the mature recirculating pool ( dashed line ). The MFI fold Δ is an average of at least n = 5 mice per strain shown, and * p < 0.05 compared with B6 mice. b The left panel shows the total numbers of AA4.1+ pro-/pre- and immature B cells in the BM of B6 ( n = 12; black bar ) and NOD ( n = 10; white bar ) and NZB/W F1 ( n = 5, gray bar ) mice. The middle panel shows the total number AA4.1+ TR B cells in the spleen of B6 ( n = 9; black bar ), NOD ( n = 9; white bar ), and NZB/W F1 ( n = 5; gray bar ) mice. The right panel shows the percent AA4.1+ cells in the pro-/pre- and immature B cells in the BM and the TR B-cell compartments in the PBL and spleen of B6 ( n = 12 for BM compartments, n = 9 for spleen, n = 5 for PBL), NOD ( n = 10 for BM compartments, n = 9 for spleen, n = 5 for PBL), and NZB/W F1 ( n = 5 for all compartments) mice. Error bars indicate ± SD, ** p < 0.01 compared with B6 mice. c The column on the left shows characteristic AA4.1 expression of B220 cells in the BM of a B6, NOD, and NZB/W F1 mouse. On the right , AA4.1 and 493 Abs are used in conjunction on B220+ gated lymphocytes in the BM. The histograms show expression of <t>mAb1696</t> by pro-/pre- and immature ( black line ) in contrast with mature recirculating ( dashed line ) B cells in the BM of B6, NOD, and NZB/W F1 mice. Staining is representative of at least n = 5 per mouse strain
Mouse Antibody Mab1696, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antibody mab1696/product/GenScript corporation
Average 90 stars, based on 1 article reviews
mouse antibody mab1696 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

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CD93 expression and absolute numbers on the various B-cell developmental subsets in BM and peripheral lymphoid organs. a The first column divides lymphoid-gated B cells in the bone marrow into mature recirculating (B220 hi /IgM+), immature (B220 lo /IgM+), and pro-/pre-B cells (B220 lo /IgM−). The histograms resolve CD93 expression by AA4.1 antibody on pro-/pre- and immature B cells ( black line ) in contrast with mature recirculating B cells ( dashed line ). The ratio of the mean fluorescence indices (MFIs) of AA4.1 expression by pro-/pre- and immature B cells to the AA4.1 expression by mature recirculating B cells is shown as MFI fold Δ on the histograms. Splenic B-cell developmental subsets are divided into the marginal zone (MZ) pool (B220+ gated, IgM hi /CD21/35 hi ), FO pool (B220+ gated, IgM int /CD21/35 int ), and TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the FO pool ( dashed line ). The ratio of MFIs of AA4.1 expression by TR B cells and FO B cells is shown as MFI fold Δ . B cells in the peripheral blood are divided into the mature recirculating (B220+ gated, IgM+/CD21/35 int ) and the TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the mature recirculating pool ( dashed line ). The MFI fold Δ is an average of at least n = 5 mice per strain shown, and * p < 0.05 compared with B6 mice. b The left panel shows the total numbers of AA4.1+ pro-/pre- and immature B cells in the BM of B6 ( n = 12; black bar ) and NOD ( n = 10; white bar ) and NZB/W F1 ( n = 5, gray bar ) mice. The middle panel shows the total number AA4.1+ TR B cells in the spleen of B6 ( n = 9; black bar ), NOD ( n = 9; white bar ), and NZB/W F1 ( n = 5; gray bar ) mice. The right panel shows the percent AA4.1+ cells in the pro-/pre- and immature B cells in the BM and the TR B-cell compartments in the PBL and spleen of B6 ( n = 12 for BM compartments, n = 9 for spleen, n = 5 for PBL), NOD ( n = 10 for BM compartments, n = 9 for spleen, n = 5 for PBL), and NZB/W F1 ( n = 5 for all compartments) mice. Error bars indicate ± SD, ** p < 0.01 compared with B6 mice. c The column on the left shows characteristic AA4.1 expression of B220 cells in the BM of a B6, NOD, and NZB/W F1 mouse. On the right , AA4.1 and 493 Abs are used in conjunction on B220+ gated lymphocytes in the BM. The histograms show expression of mAb1696 by pro-/pre- and immature ( black line ) in contrast with mature recirculating ( dashed line ) B cells in the BM of B6, NOD, and NZB/W F1 mice. Staining is representative of at least n = 5 per mouse strain

Journal: Immunogenetics

Article Title: A novel CD93 polymorphism in non-obese diabetic (NOD) and NZB/W F1 mice is linked to a CD4+ iNKT cell deficient state

doi: 10.1007/s00251-010-0442-3

Figure Lengend Snippet: CD93 expression and absolute numbers on the various B-cell developmental subsets in BM and peripheral lymphoid organs. a The first column divides lymphoid-gated B cells in the bone marrow into mature recirculating (B220 hi /IgM+), immature (B220 lo /IgM+), and pro-/pre-B cells (B220 lo /IgM−). The histograms resolve CD93 expression by AA4.1 antibody on pro-/pre- and immature B cells ( black line ) in contrast with mature recirculating B cells ( dashed line ). The ratio of the mean fluorescence indices (MFIs) of AA4.1 expression by pro-/pre- and immature B cells to the AA4.1 expression by mature recirculating B cells is shown as MFI fold Δ on the histograms. Splenic B-cell developmental subsets are divided into the marginal zone (MZ) pool (B220+ gated, IgM hi /CD21/35 hi ), FO pool (B220+ gated, IgM int /CD21/35 int ), and TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the FO pool ( dashed line ). The ratio of MFIs of AA4.1 expression by TR B cells and FO B cells is shown as MFI fold Δ . B cells in the peripheral blood are divided into the mature recirculating (B220+ gated, IgM+/CD21/35 int ) and the TR pool (B220+ gated, IgM+/CD21/35 lo ). The histograms display AA4.1 expression by the TR pool ( black line ) in comparison with the mature recirculating pool ( dashed line ). The MFI fold Δ is an average of at least n = 5 mice per strain shown, and * p < 0.05 compared with B6 mice. b The left panel shows the total numbers of AA4.1+ pro-/pre- and immature B cells in the BM of B6 ( n = 12; black bar ) and NOD ( n = 10; white bar ) and NZB/W F1 ( n = 5, gray bar ) mice. The middle panel shows the total number AA4.1+ TR B cells in the spleen of B6 ( n = 9; black bar ), NOD ( n = 9; white bar ), and NZB/W F1 ( n = 5; gray bar ) mice. The right panel shows the percent AA4.1+ cells in the pro-/pre- and immature B cells in the BM and the TR B-cell compartments in the PBL and spleen of B6 ( n = 12 for BM compartments, n = 9 for spleen, n = 5 for PBL), NOD ( n = 10 for BM compartments, n = 9 for spleen, n = 5 for PBL), and NZB/W F1 ( n = 5 for all compartments) mice. Error bars indicate ± SD, ** p < 0.01 compared with B6 mice. c The column on the left shows characteristic AA4.1 expression of B220 cells in the BM of a B6, NOD, and NZB/W F1 mouse. On the right , AA4.1 and 493 Abs are used in conjunction on B220+ gated lymphocytes in the BM. The histograms show expression of mAb1696 by pro-/pre- and immature ( black line ) in contrast with mature recirculating ( dashed line ) B cells in the BM of B6, NOD, and NZB/W F1 mice. Staining is representative of at least n = 5 per mouse strain

Article Snippet: The antibodies used in this study were as follows: PerCP conjugated anti-CD45R (B220, RA3-6B2), APC conjugated anti-IgM (II/41), FITC/PE conjugated anti-CD21/35 (7G6), PE conjugated anti-AA4.1, biotinylated 493 mAb (BD Biosciences, San Diego, CA), mAb1696 (R&D systems, Minneapolis, MN), and anti-Rat IgG2b (RG7/11.1) (BD Biosciences).

Techniques: Expressing, Fluorescence, Comparison, Staining